SCIENTIFIC SERVICES PLATFORMS

Citomics

Flow Cytometry Laboratory

Technical staff

Marta Soler
E-mail: MSolerC@santpau.cat / citometria@santpau.cat

Functions

Convencional cytometry

In homogeneous samples (cell lines) or heterogeneous samples (biologic liquids):

  • Detection of proteins and determination of cell populations (cell markers).
  • Studies of signalling cascades (cell cycle, cell proliferation, apoptosis, intracellular calcium determination).
  • Quantification of transfection efficiency.
  • Phenotype changes. Relative and absolute presence.
  • Production of mediators.
  • Detection of minority populations.

Cell sorter

Cell subpopulations

Equipment

  • FACSCalibur (BD) conventional flow cytometry.
  • MACSQuant (Miltenyi Biotech) conventional flow cytometry.
  • FACS (BD) flow cytometry with cell sorting.

Grants

  • IRHSCSP. “Flow cytometry cell sorter: analysis system and high throughput cell sorting”. IF06/3678-1. Co-funding: FEDER.

Cell Cultures Laboratory

Technical staff

Elena Serrano
E-mail: eserrano@santpau.cat

Functions

To make suitable facilities and equipment available to researchers working with cell cultures in a BSL2 laboratory.

Equipment

  • 4 CO2 incubators
  • 2 hypoxia incubators (2-21% O2)
  • 2 IIA biosafety cabinets (BSL2)
  • 2 hypoxia cabinets (BSL1)
  • Culture baths
  • Olympus inverted microscope with high-resolution colour camera
  • Refrigerated centrifuge
  • 4ºC refrigerator and -20ºC freezer
  • Thermo-desinfecter and autoclave.

Forms

Cytometry service form

Cultures service form

Last update: 14 de December de 2016

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