Presentation
3 services for both external and internal customers:
- Biochemical analysis in biofluids by an autoanalyzer (COBAS c501/c6000).
- Quantitative and functional characterization of lipoproteins.
- Preparation and sale of human native and modified lipoproteins.
Services
The services that we offer are:
1. Determination of biochemical parameters of samples (cell supernatants, serum, plasma, urine) by autoanalyzer (COBAS c501/c6000):
- Apo A-I
- Apo B100
- Apo E
- Apo A-II
- Apo C-II
- Apo C-III
- Albumin
- Alkaline phosphatase
- Alanine aminotransferase (ALT) (GPT)
- Amylase
- Aspartate aminotransferase (AST) (GOT)
- Bilirubin
- Cholesterol (total, LDL and HDL cholesterol)
- Creatine kinase (CK)
- Creatinine
- C-reactive protein
- D-dimer
- Ferritin
- Fructosamine
- Free fatty acids (FFA)
- Gamma glutamyl transferase (GGT)
- Glucose
- Glycerol
- Haptoglobin
- HbA1c
- Lactate deshidrogenase (LDH)
- Lipase
- Lipoprotein (a)
- Transferrin
- Tryglicerides
- Urea
- Urine proteins
2. Functional characterization of plasma lipoproteins:
- Antioxidant ability of HDL
- Lipoprotein-associated phospholipase A2 activity (total or in lipoproteins)
- LDL subfractions
- Paraoxonase 1 activity
- Susceptibility to oxidation (LDL and HDL)
- VLDL, LDL, and HDL composition (mice and human)
3. Supply of purified lipoproteins (native, modified or labeled) for research:
- ApoA-I (free)
- Acetylated LDL
- DiI-LDL
- Electronegative LDL
- Glycated LDL
- Oxidized LDL
- VLDL (d < 1.006 mg/ml)
- IDL (1.006-1.019)
- LDL (1.019-1.063)
- LDL (1.019-1.050)
- HDL (1.063-1.210)
Experience and Trajectory
The lead investigators of this initiative have extensive years of experience studying lipid and lipoprotein metabolism.
Research Projects:
- ISCIII PI17/00232, PI16/00139, PI16/00471
- Fundació La Marató 2016, 201602.31
- Ministry of Science, Innovation and Universities (PID2019-104367RB-100)
Publications:
- Changes in the Composition and Function of Lipoproteins after Bariatric Surgery in Patients with Severe Obesity. Genua I, et al. J Clin Med. 2021 Apr
16;10(8):1716. - LDL Receptor Regulates the Reverse Transport of Macrophage-Derived Unesterified Cholesterol via Concerted Action of the HDL-LDL Axis: Insight From Mouse Models. Cedó L, et al. Circ Res. 2020 Aug 28;127(6):778-792.
- Familial Combined Hyperlipidemia (FCH) Patients with High Triglyceride Levels Present with Worse Lipoprotein Function Than FCH Patients with Isolated Hypercholesterolemia. Puig N, et al. Biomedicines. 2020 Jan 6;8(1):6.
- Subcutaneous Administration of Apolipoprotein J-Derived Mimetic Peptide d-[113-122]apoJ Improves LDL and HDL Function and Prevents Atherosclerosis in LDLR-KO Mice. Rivas-Urbina A, et al. Biomolecules. 2020 May 29;10(6):829.
- Nicotinamide Prevents Apolipoprotein B-Containing Lipoprotein Oxidation, Inflammation and Atherosclerosis in Apolipoprotein E-Deficient Mice. Méndez-Lara KA, et al. Antioxidants (Basel). 2020 Nov 21;9(11):1162.
Equipment
Equipment involved in providing the service:
- Cobas 6000/c501 Autoanalyzer (Hitachi)
- Synergy HT Fluorimeter (Biotek)
- NGC Chromatography System (BioRad)
Responsibles
Contact mail: jsanche
Scientific Responsible
Technical Responsible
Manager Responsible
