Sant Pau - Institut de Recerca

Principal research lines

Development of fluid biomarkers of synapse degeneration in neurodegenerative diseases and neuropsychiatric disorders.
-omic approaches to understand the molecular basis of synapse degeneration in Alzheimer’s disease.
Pathway-based polygenic risk scores to elucidate the genetic factors underlying Alzheimer’s disease and use of in vitro cell-based assays to assess their function.

Principals Techniques/Expertise

Analysis of proteomic data (SRM/MRM/PRM, LFQ) by PCA, WGCNA, PathFindR, regressions and correlations.
Technical and clinical validation of antibodies and immunoassays in postmortem brain tissue (WB, IHC) and patient-derived biofluids (ELISA/SIMOA).
microRNAomics including miRNA panels and targeted assays.
Synaptosome fractionation from postmortem tissue and isolation of extracellular vesicles.
Massively parallel reporter assays (molecular cloning and in vitro assays).
Multimodal correlative analyses in clinical cohorts.
Fourier-transform infrared spectroscopy analysis in postmortem tissue and patient-derived biofluids.
Pathway-based generation of polygenic risk scores.
nlme modeling of cognitive decline related to genetic risk factors.

Group coordination

BELBIN, OLIVIA (IR)
OBelbin@santpau.cat

Members

Cervantes Gonzalez, Alba IR
Lidon Gil, Laia IR
Perlaza Meneses, Danna A. IR
Serrano Requena, Sara IR

Publications 2022

(JIF 2022)

Delaby C, Alcolea D, Hirtz C, Vialaret J, Kindermans J, Morichon L, Fortea J, Belbin O, Gabelle A, Blennow K, Zetterberg H, Lleo A, Lehmann S. Blood amyloid and tau biomarkers as predictors of cerebrospinal fluid profiles. JOURNAL OF NEURAL TRANSMISSION. 2022; 129(2). DOI:10.1007/s00702-022-02474-9. PMID:35169889. IF:3,3 (Q2/5D). Document type: Article.
Delaby C, Estelles T, Zhu NU, Arranz J, Barroeta I, Carmona M, Illan I, Santos MA, Altuna M, Sala I, Sanchez MB, Videla L, Valldeneu S, Subirana A, Tondo M, Blanco F, Lehmann S, Belbin O, Blesa R, Fortea J, Lleo A, Alcolea D. The A beta 1-42/A beta 1-40 ratio in CSF is more strongly associated to tau markers and clinical progression than A beta 1-42 alone. Alzheimers Research & Therapy. 2022; 14(1):20. DOI:10.1186/s13195-022-00967-z. PMID:35105351. IF:9 (Q1/1D). Document type: Article.
Gómez de San José N, Goossens J, Al Shweiki MR, Halbgebauer S, Oeckl P, Steinacker P, Danzer KM, Graf H, Schönfeldt C, Belbin O, Lleó A, Vanmechelen E, Otto M. Glutamate receptor 4 as a fluid biomarker for the diagnosis of psychiatric disorders. JOURNAL OF PSYCHIATRIC RESEARCH. 2022; 156. DOI:10.1016/j.jpsychires.2022.10.010. PMID:36323141. IF:4,8 (Q2/4D). Document type: Article.
Gonzalez AC, Belbin O. Fluid markers of synapse degeneration in synucleinopathies. JOURNAL OF NEURAL TRANSMISSION. 2022; 129(2). DOI:10.1007/s00702-022-02467-8. PMID:35147800. IF:3,3 (Q2/5D). Document type: Review.
Gonzalez AC, Irwin DJ, Alcolea D, McMillan CT, Chen A, Wolk D, Sirisi S, Dols O, Querol M, Illan I, Santos MA, Fortea J, Lee EB, Trojanowski JQ, Grossman M, Lleo A, Belbin O. Multimarker synaptic protein cerebrospinal fluid panels reflect TDP-43 pathology and cognitive performance in a pathological cohort of frontotemporal lobar degeneration. Molecular Neurodegeneration. 2022; 17(1):29. DOI:10.1186/s13024-022-00534-y. PMID:35395770. IF:15,1 (Q1/1D). Document type: Article.
Illan I, Montal V, Borrego S, Mandelli ML, Falgas N, Welch AE, Pegueroles J, Santos M, Bejanin A, Alcolea D, Dols O, Belbin O, Sanchez MB, Bargallo N, Gonzalez S, Llado A, Blesa R, Dickerson BC, Rosen HJ, Miller BL, Lleo A, Gorno ML, Sanchez R, Fortea J. Cortical microstructure in primary progressive aphasia: a multicenter study. Alzheimers Research & Therapy. 2022; 14(1):27. DOI:10.1186/s13195-022-00974-0. PMID:35139897. IF:9 (Q1/1D). Document type: Article.
Iulita MF, Chavez DG, Christensen MK, Tamayo NV, Plana O, Rasmussen SA, Figuls MR, Alcolea D, Videla L, Barroeta I, Benejam B, Altuna M, Padilla C, Pegueroles J, Fernandez S, Belbin O, Carmona M, Blesa R, Lleo A, Bejanin A, Fortea J. Association of Alzheimer Disease with Life Expectancy in People with Down Syndrome. JAMA Network Open. 2022; 5(5):e2212910. DOI:10.1001/jamanetworkopen.2022.12910. PMID:35604690. IF:13,8 (Q1/1D). Document type: Article.
Jansen RE, van der Lee SJ, Gomez D, de Rojas I, Dalmasso MC, Grenier B, Zettergren A, Mishra A, Ali M, Andrade V, Bellenguez C, Kleineidam L, Kucukali F, Sung YJ, Tesi N, Vromen EM, Wightman DP, Alcolea D, Alegret M, Alvarez I, Amouyel P, Athanasiu L, Bahrami S, Bailly H, Belbin O, Bergh S, Bertram L, Biessels GJ, Blennow K, Blesa R, Boada M, Boland A, Buerger K, et al. [Dols-Icardo O, Fortea J, Lleo A]. Genome-wide meta-analysis for Alzheimer’s disease cerebrospinal fluid biomarkers. ACTA NEUROPATHOLOGICA. 2022; 144(5). DOI:10.1007/s00401-022-02454-z. PMID:36066633. IF:12,7 (Q1/1D). Document type: Article.
Querol M, Sirisi S, Molina L, Molina B, Pegueroles J, Ferrer P, Nunez R, Dols O, Balasa M, Iulita MF, Blesa R, Belbin O, Clarimon J, Fortea J, Gelpi E, Sanchez R, Lleo A. Neuropathology of a patient with Alzheimer disease treated with low doses of verubecestat. NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY. 2022; 48(3):e12781. DOI:10.1111/nan.12781. PMID:34825396. IF:5 (Q1/2D). Document type: Article.
Reyes D, Dols O, Sirisi S, Cortes E, Turon J, De Luna N, Blesa R, Belbin O, Montal V, Alcolea D, Fortea J, Lleo A, Rojas R, Illan I. Pathophysiological Underpinnings of Extra-Motor Neurodegeneration in Amyotrophic Lateral Sclerosis: New Insights From Biomarker Studies. Frontiers in Neurology. 2022; 12:750543. DOI:10.3389/fneur.2021.750543. PMID:35115992. IF:3,4 (Q2/5D). Document type: Review.
Sirisi S, Querol M, Dols O, Pegueroles J, Montal V, Munoz L, Torres S, Ferrer P, Iulita MF, Sanchez E, Blesa R, Illan I, Molina L, Borrego S, Sanchez R, Clarimon J, Belbin O, Fortea J, Lleo A. Myelin loss in C9orf72 hexanucleotide expansion carriers. JOURNAL OF NEUROSCIENCE RESEARCH. 2022; 100(10). DOI:10.1002/jnr.25100. PMID:35766328. IF:4,2 (Q2/4D). Document type: Article.
Valle N, Pérez R, Chiva G, Belbin O, Serrano S, Sirisi S, González AC, Giró O, Sánchez É, Dols O, Alcolea D, Carmona M, Jimenez A, Lleó A, Fortea J, Iulita MF. Enrichment of Astrocyte-Derived Extracellular Vesicles from Human Plasma. Jove-Journal of Visualized Experiments. 2022; 2022(186):e64107. DOI:10.3791/64107. PMID:35993755. IF:1,2 (Q3/8D). Document type: Article.

Introduction

Principal research lines

Development of fluid biomarkers of synapse degeneration in neurodegenerative diseases and neuropsychiatric disorders.
-omic approaches to understand the molecular basis of synapse degeneration in Alzheimer’s disease.
Pathway-based polygenic risk scores to elucidate the genetic factors underlying Alzheimer’s disease and use of in vitro cell-based assays to assess their function.

Principals Techniques/Expertise

Analysis of proteomic data (SRM/MRM/PRM, LFQ) by PCA, WGCNA, PathFindR, regressions and correlations.
Technical and clinical validation of antibodies and immunoassays in postmortem brain tissue (WB, IHC) and patient-derived biofluids (ELISA/SIMOA).
microRNAomics including miRNA panels and targeted assays.
Synaptosome fractionation from postmortem tissue and isolation of extracellular vesicles.
Massively parallel reporter assays (molecular cloning and in vitro assays).
Multimodal correlative analyses in clinical cohorts.
Fourier-transform infrared spectroscopy analysis in postmortem tissue and patient-derived biofluids.
Pathway-based generation of polygenic risk scores.
nlme modeling of cognitive decline related to genetic risk factors.

Team

Group coordination

BELBIN, OLIVIA (IR)
OBelbin@santpau.cat

Members

Cervantes Gonzalez, Alba IR
Lidon Gil, Laia IR
Perlaza Meneses, Danna A. IR
Serrano Requena, Sara IR

Scientific output

Publications 2022

(JIF 2022)

Delaby C, Alcolea D, Hirtz C, Vialaret J, Kindermans J, Morichon L, Fortea J, Belbin O, Gabelle A, Blennow K, Zetterberg H, Lleo A, Lehmann S. Blood amyloid and tau biomarkers as predictors of cerebrospinal fluid profiles. JOURNAL OF NEURAL TRANSMISSION. 2022; 129(2). DOI:10.1007/s00702-022-02474-9. PMID:35169889. IF:3,3 (Q2/5D). Document type: Article.
Delaby C, Estelles T, Zhu NU, Arranz J, Barroeta I, Carmona M, Illan I, Santos MA, Altuna M, Sala I, Sanchez MB, Videla L, Valldeneu S, Subirana A, Tondo M, Blanco F, Lehmann S, Belbin O, Blesa R, Fortea J, Lleo A, Alcolea D. The A beta 1-42/A beta 1-40 ratio in CSF is more strongly associated to tau markers and clinical progression than A beta 1-42 alone. Alzheimers Research & Therapy. 2022; 14(1):20. DOI:10.1186/s13195-022-00967-z. PMID:35105351. IF:9 (Q1/1D). Document type: Article.
Gómez de San José N, Goossens J, Al Shweiki MR, Halbgebauer S, Oeckl P, Steinacker P, Danzer KM, Graf H, Schönfeldt C, Belbin O, Lleó A, Vanmechelen E, Otto M. Glutamate receptor 4 as a fluid biomarker for the diagnosis of psychiatric disorders. JOURNAL OF PSYCHIATRIC RESEARCH. 2022; 156. DOI:10.1016/j.jpsychires.2022.10.010. PMID:36323141. IF:4,8 (Q2/4D). Document type: Article.
Gonzalez AC, Belbin O. Fluid markers of synapse degeneration in synucleinopathies. JOURNAL OF NEURAL TRANSMISSION. 2022; 129(2). DOI:10.1007/s00702-022-02467-8. PMID:35147800. IF:3,3 (Q2/5D). Document type: Review.
Gonzalez AC, Irwin DJ, Alcolea D, McMillan CT, Chen A, Wolk D, Sirisi S, Dols O, Querol M, Illan I, Santos MA, Fortea J, Lee EB, Trojanowski JQ, Grossman M, Lleo A, Belbin O. Multimarker synaptic protein cerebrospinal fluid panels reflect TDP-43 pathology and cognitive performance in a pathological cohort of frontotemporal lobar degeneration. Molecular Neurodegeneration. 2022; 17(1):29. DOI:10.1186/s13024-022-00534-y. PMID:35395770. IF:15,1 (Q1/1D). Document type: Article.
Illan I, Montal V, Borrego S, Mandelli ML, Falgas N, Welch AE, Pegueroles J, Santos M, Bejanin A, Alcolea D, Dols O, Belbin O, Sanchez MB, Bargallo N, Gonzalez S, Llado A, Blesa R, Dickerson BC, Rosen HJ, Miller BL, Lleo A, Gorno ML, Sanchez R, Fortea J. Cortical microstructure in primary progressive aphasia: a multicenter study. Alzheimers Research & Therapy. 2022; 14(1):27. DOI:10.1186/s13195-022-00974-0. PMID:35139897. IF:9 (Q1/1D). Document type: Article.
Iulita MF, Chavez DG, Christensen MK, Tamayo NV, Plana O, Rasmussen SA, Figuls MR, Alcolea D, Videla L, Barroeta I, Benejam B, Altuna M, Padilla C, Pegueroles J, Fernandez S, Belbin O, Carmona M, Blesa R, Lleo A, Bejanin A, Fortea J. Association of Alzheimer Disease with Life Expectancy in People with Down Syndrome. JAMA Network Open. 2022; 5(5):e2212910. DOI:10.1001/jamanetworkopen.2022.12910. PMID:35604690. IF:13,8 (Q1/1D). Document type: Article.
Jansen RE, van der Lee SJ, Gomez D, de Rojas I, Dalmasso MC, Grenier B, Zettergren A, Mishra A, Ali M, Andrade V, Bellenguez C, Kleineidam L, Kucukali F, Sung YJ, Tesi N, Vromen EM, Wightman DP, Alcolea D, Alegret M, Alvarez I, Amouyel P, Athanasiu L, Bahrami S, Bailly H, Belbin O, Bergh S, Bertram L, Biessels GJ, Blennow K, Blesa R, Boada M, Boland A, Buerger K, et al. [Dols-Icardo O, Fortea J, Lleo A]. Genome-wide meta-analysis for Alzheimer’s disease cerebrospinal fluid biomarkers. ACTA NEUROPATHOLOGICA. 2022; 144(5). DOI:10.1007/s00401-022-02454-z. PMID:36066633. IF:12,7 (Q1/1D). Document type: Article.
Querol M, Sirisi S, Molina L, Molina B, Pegueroles J, Ferrer P, Nunez R, Dols O, Balasa M, Iulita MF, Blesa R, Belbin O, Clarimon J, Fortea J, Gelpi E, Sanchez R, Lleo A. Neuropathology of a patient with Alzheimer disease treated with low doses of verubecestat. NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY. 2022; 48(3):e12781. DOI:10.1111/nan.12781. PMID:34825396. IF:5 (Q1/2D). Document type: Article.
Reyes D, Dols O, Sirisi S, Cortes E, Turon J, De Luna N, Blesa R, Belbin O, Montal V, Alcolea D, Fortea J, Lleo A, Rojas R, Illan I. Pathophysiological Underpinnings of Extra-Motor Neurodegeneration in Amyotrophic Lateral Sclerosis: New Insights From Biomarker Studies. Frontiers in Neurology. 2022; 12:750543. DOI:10.3389/fneur.2021.750543. PMID:35115992. IF:3,4 (Q2/5D). Document type: Review.
Sirisi S, Querol M, Dols O, Pegueroles J, Montal V, Munoz L, Torres S, Ferrer P, Iulita MF, Sanchez E, Blesa R, Illan I, Molina L, Borrego S, Sanchez R, Clarimon J, Belbin O, Fortea J, Lleo A. Myelin loss in C9orf72 hexanucleotide expansion carriers. JOURNAL OF NEUROSCIENCE RESEARCH. 2022; 100(10). DOI:10.1002/jnr.25100. PMID:35766328. IF:4,2 (Q2/4D). Document type: Article.
Valle N, Pérez R, Chiva G, Belbin O, Serrano S, Sirisi S, González AC, Giró O, Sánchez É, Dols O, Alcolea D, Carmona M, Jimenez A, Lleó A, Fortea J, Iulita MF. Enrichment of Astrocyte-Derived Extracellular Vesicles from Human Plasma. Jove-Journal of Visualized Experiments. 2022; 2022(186):e64107. DOI:10.3791/64107. PMID:35993755. IF:1,2 (Q3/8D). Document type: Article.

NEUROLOGICAL DISEASES, NEUROSCIENCE & MENTAL HEALTH

Molecular Neurodegeneration

Principal research lines

Principals Techniques/Expertise

Group coordination

Members

Publications 2022

Principal research lines

Principals Techniques/Expertise

Group coordination

Members

Publications 2022

Institut de Recerca de l’Hospital de la Santa Creu i Sant Pau